anti integrin beta1  (Developmental Studies Hybridoma Bank)

Journal: bioRxiv

Article Title: Integrin Activation Enhances Lesion-Specific Targeting of Monocyte-Mimetic Nanoparticles in Atherosclerosis

doi: 10.64898/2026.03.04.707824

Figure Lengend Snippet: Fluorescence images and quantification of nanoparticle uptake in TNFα-activated ECs under the following conditions: (A) VCAM1 blockade, (B) β1-integrin blockade on nanoparticles, and (C) CPZ treatment. Red: IA@MoNP, MoNP, or bare NP; blue: DAPI-stained nuclei. Scale bar = 50 μm. (A–B) *p < 0.05 vs. IgG antibody; # p < 0.05 vs. MoNP. (C) *p < 0.05 vs. control ECs; # p < 0.05 vs. bare NP. Data are presented as mean ± SD from n = 3 independent experiments.

Article Snippet: Membrane proteins were assessed by Western blot using antibodies against CD11b (Cell Signaling #17800, 1:1000), Na + /K + ATPase (Cell Signaling #3010, 1:1000), α4-integrin (Invitrogen #PA5-20599, 1:1000), and β1-integrin (Cell Signaling #4706S, 1:1000).

Techniques: Fluorescence, Staining, Control

Journal: bioRxiv

Article Title: Integrin Activation Enhances Lesion-Specific Targeting of Monocyte-Mimetic Nanoparticles in Atherosclerosis

doi: 10.64898/2026.03.04.707824

Figure Lengend Snippet: (A) Schematic illustration of the experimental design. (B) IVIS imaging and quantification of nanoparticle accumulation in partially ligated LCA of ApoE -/- mice. (C) Representative cross-sectional fluorescence images of the LCA. Red: IA@MoNP or MoNP; green: elastin fibers; blue: DAPI-stained nuclei. Scale bar = 100 μm. (D–E) IVIS analysis showing (D) biodistribution in major organs across nanoparticle formulations and (E) reduction of IA@MoNP signal in the LCA following β1-integrin blockade on nanoparticles. (B) *p < 0.05 vs. RCA; # p < 0.05 vs. MoNP; (E) *p < 0.05 vs. IgG antibody. Data are presented as mean ± SD from n = 4 mice each group.

Article Snippet: Membrane proteins were assessed by Western blot using antibodies against CD11b (Cell Signaling #17800, 1:1000), Na + /K + ATPase (Cell Signaling #3010, 1:1000), α4-integrin (Invitrogen #PA5-20599, 1:1000), and β1-integrin (Cell Signaling #4706S, 1:1000).

Techniques: Imaging, Fluorescence, Staining

Journal: Journal of Orthopaedic Translation

Article Title: Mechanotransduction-driven macrophage polarization via Integrin-SRC-STAT6 pathway in distraction osteogenesis

doi: 10.1016/j.jot.2025.10.016

Figure Lengend Snippet: Integrin-β/SRC/STAT6 signaling pathway mediates mechanical stimulation-induced macrophage polarization. (A) Relative protein levels of Integrin-β, total SRC, total STAT6 in the NC and ST group. (B) Levels of phosphorylation of SRC and STAT6 at different time points. (C) Relative expression levels of p-STAT6 and Tubulin, and their subcellular localization. (D) Semi-quantitative statistical analysis of fluorescence intensity of p-STAT6 and Tubulin. (E) Relative protein levels of Arg-1, Integrin-β, total SRC, p-SRC, total STAT6 and p-STAT6. (F) Flow cytometry data showing the percentage of CD206 − CD86 + and CD86 + CD206 − cells in the NC and ST,Saracatinib and ST + Saracatinib group. n = 4, ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001 by unpaired Students’-t test and one-way ANOVA and Tukey's post hoc tests.

Article Snippet: The PVDF membrane with blots was blocked in the tris-buffered saline with 5 %(w/v) skim milk powder at room temperature for 1 h. And then, Incubated the membrane with the primary antibody against the target protein overnight at 4 °C, which included iNOS (1:1000,ab15323, Abcam, Cambridge, UK), Integrin-β (1:1000, #4706, Cell Signaling Technology, Danvers, USA), STAT6 (1:1000, # 5397, Cell Signaling Technology, Danvers, USA), p-STAT6 (1:1000, #56554, Cell Signaling Technology, Danvers, USA), SRC (1:1000, #2109, Cell Signaling Technology, Danvers, USA), p-SRC (1:1000, #6943, Cell Signaling Technology, Danvers, USA), Arg-1 (1:500, PTM-5730, PTMBio, Hangzhou, China), GAPDH (1:5000, GB11002, Servicebio, Wuhan, China), Col-1 (1:1000, ab88147, Abcam, Cambridge, UK), Runx-2 (1:1000, #12556, Cell Signaling Technology, Danvers, USA), OCN (1:1000, AF6300, Beyotime, Shanghai, China).

Techniques: Phospho-proteomics, Expressing, Fluorescence, Flow Cytometry